Area of Study 1:  Biotechnology

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Outcome:
  • Evaluate how tools and techniques can be used and applied in the manipulation of DNA
  • Evaluate how biotechnology can be used to solve problems related to the regulation of biochemical pathways


DNA manipulation techniques and applications

  • The use of enzymes to manipulate DNA, including polymerase to synthesise DNA, ligase to join DNA and endonucleases to cut DNA
  • The function of CRISPR-Cas9 in bacteria and the application of this function in editing an organism’s genome
  • Amplification of DNA using polymerase chain reaction
  • The use of gel electrophoresis in sorting DNA fragments, including the interpretation of gel runs for DNA profiling
  • The use of recombinant plasmids as vectors to transform bacterial cells as demonstrated by the production of human insulin
  • The use of genetically modified and transgenic organisms in agriculture to increase crop productivity and to provide resistance to disease


Biotechnological applications of biochemical pathways

  • Potential uses and applications of CRISPR-Cas9 technologies to improve photosynthetic efficiencies and crop yields


Quick Links:

Core Technologies

Genetic Modification

Gene Cloning

Gene Editing